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Classes of Endonucleases

APBIO-4VTCK2

Restriction endonucleases will make two types of cuts: those that result in blunt ends (A) and those that result in ends with overhangs of one strand (B).

Based on the chemical characteristics of the fragment ends, which of the following predicts which type of digest is more likely to happen?

(A)

"Restriction Enzyme." Wikipedia. Wikimedia Foundation, n.d. Web. 05 Mar. 2016. https://en.wikipedia.org/wiki/Restriction_enzyme.

(B)

"Restriction Enzyme." Wikipedia. Wikimedia Foundation, n.d. Web. 05 Mar. 2016. https://en.wikipedia.org/wiki/Restriction_enzyme.

A

The blunt fragments are more likely because the re-annealing process is a seamless connection.

B

The blunt fragments are more likely because of complementarity of the adjoining fragments.

C

The overhang ends are more likely because the overhangs fit like two puzzle pieces.

D

The overhang ends are more likely because of base complementarity leading to optimal hydrogen bonding.