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Difficult

Enzyme Inquiry Lab

APBIO-VKWV1G

Four AP Biology students working in a team were asked to design a lab to test the effect of an environmental factor that affects the enzyme activity of catalase on the rate of hydrogen peroxide decomposition. Each student came up with a different idea.

Upon collaboration, the one idea that was eliminated as a valid possibility was

A

adding different pH buffers from 1 through 12 to the hydrogen peroxide solution.

B

changing the temperature of the hydrogen peroxide from 10 °C to 65 °C at 5 °C intervals.

C

adding different concentrations of $NaCl$ from 0 M to 1.5 M at 0.2 M intervals to the hydrogen peroxide solution.

D

adding different enzymes in addition to catalase to the hydrogen peroxide solution including sucrase and lipase.