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Moderate

GFP Expression

APBIO-NEW2KK

One can use DNA transformation as a laboratory method to study regulation of gene expression. One such system frequently used in high school biology is the genetically engineered plasmid, pGLO, which incorporates parts of the arabinose operon to drive the expression of green fluorescent protein (GFP).

Which of the following charts predicts the GFP expression for the bacteria plated under the conditions described?

A
Plate 1 Plate 2 Plate 3 Plate 4 Plate 5
LB w/o pGLO LB/AMP w/o pGLO LB/AMP w/ pGLO LB/AMP/ara w/o pGLO LB/AMP/ara w/ pGLO
Yes No Yes No Yes
B
Plate 1 Plate 2 Plate 3 Plate 4 Plate 5
LB w/o pGLO LB/AMP w/o pGLO LB/AMP w/ pGLO LB/AMP/ara w/o pGLO LB/AMP/ara w/ pGLO
No No No No No
C
Plate 1 Plate 2 Plate 3 Plate 4 Plate 5
LB w/o pGLO LB/AMP w/o pGLO LB/AMP w/ pGLO LB/AMP/ara w/o pGLO LB/AMP/ara w/ pGLO
No No No No Yes
D
Plate 1 Plate 2 Plate 3 Plate 4 Plate 5
LB w/o pGLO LB/AMP w/o pGLO LB/AMP w/ pGLO LB/AMP/ara w/o pGLO LB/AMP/ara w/ pGLO
Yes No No Yes Yes