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Moderate

Transformation Using AmpR

APBIO-JV6CVT

In a set of transformation experiments, competent E. coli cells were incubated in buffer with and without pGLO and then plated on LB agar (LB) in the presence or absence of ampicillin (AMP) and arabinose (ara). After overnight incubation, plates were examined for colony formation.

Which of the following predicts the pattern of colony formation?

A
Plate 1 Plate 2 Plate 3 Plate 4 Plate 5
Conditions LB
w/o pGLO
LB/AMP
w/o pGLO
LB/AMP
w/ pGLO
LB/AMP/ara
w/o pGLO
LB/AMP/ara
w/ pGLO
Colonies Yes No Yes No Yes
B
Plate 1 Plate 2 Plate 3 Plate 4 Plate 5
Conditions LB
w/o pGLO
LB/AMP
w/o pGLO
LB/AMP
w/ pGLO
LB/AMP/ara
w/o pGLO
LB/AMP/ara
w/ pGLO
Colonies No No Yes No Yes
C
Plate 1 Plate 2 Plate 3 Plate 4 Plate 5
Conditions LB
w/o pGLO
LB/AMP
w/o pGLO
LB/AMP
w/ pGLO
LB/AMP/ara
w/o pGLO
LB/AMP/ara
w/ pGLO
Colonies Yes No No No No
D
Plate 1 Plate 2 Plate 3 Plate 4 Plate 5
Conditions LB
w/o pGLO
LB/AMP
w/o pGLO
LB/AMP
w/ pGLO
LB/AMP/ara
w/o pGLO
LB/AMP/ara
w/ pGLO
Colonies Yes Yes No Yes No