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Biochemistry

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Designing an ELISA

BIOCHM-BKHK6M

Due to poor vaccination rates in certain parts of the United States, outbreaks of easily preventable diseases - such as measles - have recently occurred. Imagine that you are a primary care physician in a poorly vaccinated area.

You wish to determine whether an immunocompromised patient with a poorly documented medical history has previously received the measles-mumps-rubella (MMR) vaccine.

Which of the following experimental designs would BEST measure your patient's antibody response to measles?

A

Plate coated with anti-IgG $\rightarrow$ load patient serum $\rightarrow$ detect with an anti-IgG-HRP antibody of a different clone.

B

Plate coated with measles antigen $\rightarrow$ block the plate $\rightarrow$ load patient serum $\rightarrow$ detect with an anti-IgG/anti-IgM/anti-IgA polyclonal HRP-conjugated antibody.

C

Coat plate with patient serum $\rightarrow$ detect measles antibodies with a measles-anti-IgG conjugated reagent.

D

Coat plate with anti-IgG $\rightarrow$ block the plate $\rightarrow$ load patient serum $\rightarrow$ load measles antigen conjugated to HRP.

E

Plate coated with measles antigen $\rightarrow$ load patient serum $\rightarrow$ detect with an anti-IgG/anti-IgM/anti-IgA polyclonal HRP-conjugated antibody.