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Biochemistry

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Effect of Changing Concentration on Enzyme Assay

BIOCHM-OICAFG

Arginase is an enzyme that is sometimes used to test for the level of kidney function. The assay calls for mixing the enzyme with manganese chloride and 0.5 ml of 0.1 M arginine solution and glycine buffer.

Two scientists performed the assay on the same sample of arginase but one used 0.5 ml of 1.0 M arginine instead of the 0.1 M arginine that the assay calls for; all other parts of the assay were done in the same way. Both scientists calculated the same activity.

What does this suggest about the concentration of arginine used in the assay in relation to the Michealis constant for the enzyme?

A

It suggests that the concentration of arginine used in the enzyme assay is significantly higher than the ${ K }_{ m }$ value for the enzyme.

B

It suggests that the concentration of arginine used in the enzyme assay is significantly lower than the ${ K }_{ m }$ value for the enzyme.

C

It suggests that the concentration of arginine used in the enzyme assay is equal to the ${ K }_{ m }$ value.

D

It is not possible to draw any conclusions about the ${ K }_{ m }$ value of the enzyme.