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Biochemistry

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Enzyme Assay to Detect a Heart Attack

BIOCHM-GKBVTN

Creatine kinase (CK) is one of several enzymes used to test for a heart attack using a blood sample. This enzyme would normally found within cells of heart but a heart attack causes those cells to rupture and release the contents into the blood.

The reaction for this enzyme is shown below. The units for enzyme activity are units or 1 micromole product/min. Neither creatine, creatine phosphate, ATP nor ADP absorb light in the ultraviolet or visible range.

In order to test for this enzyme by an enzyme assay, what other information is needed?

The reaction catalyzed is

$$\text{Creatine + ADP} \longrightarrow \text{Creatine phosphate + ADP}$$

A

The molecular weight of creatine is needed so the protein can be determined by its size on a gel for protein electrophoresis.

B

The cellular location of CK needs to be known so the correct location can be collected from the cell.

C

A reagent that reacts with either ADP or creatine phosphate to produce a molecule that absorbs in the ultra violet or visible range.

D

The amino acid sequence of creatine kinase needs to be known so that it can be identified by sequencing the protien.

E

The DNA sequence of the gene that encodes creatine kinase must be known.