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# Enzyme Kinetics: Determining ${ K }_{ m }$

BIOCHM-JPJZ4Q

Consider the plot below illustrating the initial velocity (${ V }_{ o }$) of a reversible enzyme reaction in the presence of increasing substrate concentration ${ [S] }$.

Which of the following BEST approximates the ${ K }_{ m }$ value?

A

0.30 ${ \mu Mmin }^{ -1 }$

B

0.15 ${ \mu Mmin }^{ -1 }$

C

400 ${ \mu M }$

D

The ${ K }_{ m }$ cannot be estimated from this type of plot.