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Biochemistry

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Moderate

Functional Roles in an Enzyme Assay

BIOCHM-MYGKZL

Arginase is an enzyme that catalyzes the formation of ornithine from arginine. When the enzyme assay is done for arginase, the instructions call for mixing the enzyme with manganese chloride for 10 minutes and then portions of an arginine solution and a glycine solution adjusted to pH 9.0 are added for 30 minutes (at which time perchloric acid is added).

Each of these components plays a different role in the enzyme assay.

Manganese chloride

, while arginine

.

The glycine pH 9.0 solution serves

, and the perchloric acid

.