Human Immunodeficiency Virus (HIV) infects and ultimately kills helper CD4+ T cells. Antiretroviral therapy (ART) can slow the growth of the virus and thus also slow disease progression, but HIV is still able to latently persist in some CD4+ T cells.
Researchers believe that a “shock and kill” approach – where these latently infected CD4+ T cells are activated and then killed by other immune cells – may be a way to augment ART and eliminate virus altogether.
You have infected helper CD4+ T cells with HIV and now want to evaluate how the expression of two transcription factors (TFs) is affected when the cells are activated with a novel histone deacetylase (HDAC) inhibitor.
You harvest the cells at various timepoints following HDAC inhibitor treatment, purify RNA from these cells and then make cDNA. You then set up a real-time quantitative PCR (qPCR) using primers for the TFs of interest, BCL6 and PRDM1. The Ct values for the qPCR are shown in the table below.
Which of the following statements best describes the experimental results?