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Biochemistry

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Moderate

Lipid Extraction Storage Protocols

BIOCHM-FBFJJN

Which of the following is NOT a good practice for the storage of tissue samples from which one wishes to extract lipids?

A

Use very fresh samples or samples that have been immediately frozen in dry ice or liquid nitrogen to avoid oxidative degradation.

B

Avoid unnecessary exposure of samples to light or air.

C

For long-term storage of samples, one can add small quantities of anti-oxidants (such as BHT or ethyl gallate) to inhibit oxidative degradation.

D

Commercial oxygen absorbers can be used to remove oxygen from samples to reduce oxidative degradation.

E

Flush storage vials and tubes with nitrogen gas to prevent oxidation.

F

All of the above are good storage protocols for tissue samples for lipid extraction.