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Which of the following is NOT a good practice for the storage of tissue samples from which one wishes to extract lipids?
Use very fresh samples or samples that have been immediately frozen in dry ice or liquid nitrogen to avoid oxidative degradation.
Avoid unnecessary exposure of samples to light or air.
For long-term storage of samples, one can add small quantities of anti-oxidants (such as BHT or ethyl gallate) to inhibit oxidative degradation.
Commercial oxygen absorbers can be used to remove oxygen from samples to reduce oxidative degradation.
Flush storage vials and tubes with nitrogen gas to prevent oxidation.
All of the above are good storage protocols for tissue samples for lipid extraction.