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Biochemistry

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Phenylalanine Hydroxylase and Isotope Effect

BIOCHM-INH@MZ

You are working on genetically engineering a microorganism, with typical protease activity, to produce phenylalanine hydroxylase (PAH). The microorganism also possesses weak/mild tyrosine decarboxylase activity.

To assess the success of your genetic engineering experiment you use basic (non-enriched) growth media and spike it with tri-phenylalanine peptide to act as the substrate and incubate the culture with heavy oxygen.

Based on your knowledge of the mechanism of PAH, identify the expected metabolite(s).

A

Tarek Mohamed. Created for Albert.io. Copyright 2016. All rights reserved.

B

Tarek Mohamed. Created for Albert.io. Copyright 2016. All rights reserved.

C

Tarek Mohamed. Created for Albert.io. Copyright 2016. All rights reserved.

D

Tarek Mohamed. Created for Albert.io. Copyright 2016. All rights reserved.

E

Tarek Mohamed. Created for Albert.io. Copyright 2016. All rights reserved.

F

Tarek Mohamed. Created for Albert.io. Copyright 2016. All rights reserved.