A colleague just performed a cellular extraction to analyze protein content.
She is particularly interested in detecting a multi-subunit, transmembrane protein with a molecular weight of ~ 45kDa (holoenzyme).
She attempted to perform electrophoresis detection assays with a standard, commercially-sourced sample of the peptide complex on four fractions from the cellular extraction protocol.
Unfortunately, her experiment did not turn out as planned (based on a previously published research study), and she seeks your advice.
Using the data provided, why was the experiment unsuccessful?
Select ALL that apply.