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Biochemistry

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Techniques to Determine Protein Localization

BIOCHM-T5VYSZ

You have identified a novel eukaryotic protein and want to determine where it localizes within the cell.

Which of the following techniques can be used to determine the location of this protein in a cell?

Select ALL that apply.

A

Immunoelectron microscopy.

B

Immunofluorescence.

C

Western Blot of whole cell extract.

D

Differential centrifugation followed by Western blotting.

E

ELISA.

F

Yeast Two-Hybrid Screen.

G

Fluorescent fusion proteins.