Biochemistry

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Thin Layer Chromatography (TLC) Characteristics of Lipids

BIOCHM-B@6J1H

Assume equal quantities of beeswax, ceramide, cholesterol, and phosphatidylinositol and sphingomyelin were dissolved in chloroform, and then subjected to thin-layer chromatography on silica gel using a developing agent composed of a mixture of chloroform, methanol and water.

Which sample would migrate farthest up the gel?

A

Beeswax

B

Ceramide

C

Cholesterol

D

Phosphotidylinositol

E

Sphingomyelin