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Biochemistry

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Use of Lectins in Purification Assays

BIOCHM-SQJM$0

Lectins are a large family of proteins that occur throughout nature. Most lectins do not have enzymatic activity but are involved in a wide array of molecular interactions.

Lectins recognize sugar moieties with a high degree of specificity. The specificity of lectins for their ligands make lectins useful as tools for glycoprotein purification.

Which of the following purification techniques makes BEST use of a lectin and its properties?

A

Affinity chromatography.

B

IEX (ion exchange chromatography).

C

Size exclusion (gel filtration) chromatography.

D

Mass spectrometry coupled with gas chromatography (GC-MS).