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Cellular and Molecular Biology

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Cell Culture: Detaching Adherent Cells

CELLS-EODJFK

Which of the following BEST describes the process of detaching adherent cells from cell culture plates for routine passaging and cell maintenance?

A

The enzyme trypsin is added to cells to digest the proteins connecting the cells to the plate.

B

A sterile cell scraper is used to gently remove the cells from the plate through mechanical force.

C

A mild detergent solution is added to the cells to disrupt the connections between the cells and the plate.

D

An acidic solution is added to the cells causing a conformational change in the proteins connecting the cells to the plate which results in cell detachment.