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Cellular and Molecular Biology

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Cell Culture: Establishing Immortalized Cell Lines

CELLS-OEUYEQ

Primary cell cultures are derived from cells or tissue taken directly from an individual, and will survive in culture for a limited number of cell divisions. Other cell cultures have been “immortalized” and will grow indefinitely in culture.

Which of the following does NOT describe a mechanism commonly used to create immortalized cell lines?

A

Artificial expression of telomerase.

B

Viral transformation.

C

Direct isolation from tumors.

D

Continuous supplement with growth factors.