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Enzyme Localization in Botanical Specimens

CELLS-UUGJML

Antibodies to ribulose-1,5-bisphosphate carboxylase/oxygenase were tagged with a red fluorescent probe and used in a plant histological experiment. Cross-sections of leaves were incubated in the antibody and subsequently examined with a fluorescent microscope.

Which cellular layer of the leaf would demonstrate the greatest fluorescence?

A

Lower epidermis

B

Spongy mesophyll

C

Palisade mesophyll

D

Vascular bundle

E

Upper epidermis