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Cellular and Molecular Biology

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Enzymes that Alter DNA

CELLS-WFG8SU

A piece of DNA is cut into fragments of varying sizes by restriction enzymes. An appropriate amount of calf-intestinal alkaline phosphatase is added to the solution.

Which ONE of the following has happened after the calf-intestinal alkaline phosphatase has been added?

A

The DNA fragments are point mutated.

B

The DNA fragments are circularized.

C

The DNA fragments have had their 5’ phosphate groups removed.

D

The DNA fragments are religated together but in a different order.

E

The DNA fragments are broken down further into individual nucleotides.