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Cellular and Molecular Biology

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Experiment to Determine Mode of Viral Infection

CELLS-IM3RPH

A new virus is infecting mucosal keratinocytes, the cells that line the oral cavity. The virus enters the cells causing the cells to slough off and the oral cavity to bleed uncontrollably.

Which of the following experiments would help to uncover the molecular mechanisms by which the virus enters the cells?

A

Infecting keratinocytes in cell culture and determining which proteins the virus interacts within the cell’s cytoplasm.

B

Mutating various extracellular receptor genes and determining the infection rate of mutated cells.

C

Infecting mice with the virus and examining antibody formation.

D

Infecting cells in culture and examining the cells by scanning electron microscopy for viral location on the cell surface.

E

Thoroughly examining the genetic makeup of the keratinocytes to determine what proteins might interact with the virus.