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Cellular and Molecular Biology

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Moderate

Genetic Engineering and Inducibility

CELLS-SDJGVF

The aberrant overexpression of oncogenes is a primary determinant of cancer development and progression. To understand the effects of a particular oncogene, it can be cloned into an expression vector and introduced into cell lines or animal models. Two important concepts in cancer development involve the level of oncogene expression and the duration of oncogene expression.

If you are studying the effects of the ras oncogene in cell lines, which of the following would you use to determine how long it needs to be expressed in order to induce cellular transformation?

A

Cre-lox system.

B

RNAi.

C

Tetracycline system.

D

Site-directed mutagenesis.