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Cellular and Molecular Biology

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Isolation of Plasmids

CELLS-ON37N5

Some bacterial cells contain plasmids, small circular DNA molecules, in addition to the larger bacterial chromosome. Usually, plasmids are easily separated from chromosomal DNA in laboratory procedures.

Why?

A

The nucleotide content of chromosomal DNA is different from that of plasmid DNA.

B

Chromosomal DNA is enclosed within a phospholipid bilayer, while plasmid DNA is not.

C

Chromosomal DNA has some protein bound to it, while plasmid DNA has very little protein.

D

Plasmid DNA is less susceptible to a chelating agent.