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Molecular Technique: Purpose of Southern Blot

CELLS-FTIVWW

Which of the following situations would call for Southern blotting?

A

Transfer of RNA from an agarose gel to a membrane for probe hybridization to study gene expression.

B

Transfer of DNA from an agarose gel to a membrane for probe hybridization.

C

Transfer of protein from a polyacrylamide gel to a membrane for detection by antibodies.

D

Transfer of protein from a polyacrylamide gel to a membrane to assess their ability to bind to DNA.