You are a scientist studying a complex developmental disorder. One day, you discover a novel candidate gene that is misregulated in patients.
Due to the complex nature of this disorder, you suspect that this novel candidate gene may be misregulated in multiple organs in various ways. A colleague informs you that you can use a Northern Blot to determine how strongly your candidate gene is expressed in different tissues.
You begin by isolating the
from patient and volunteer tissue samples.
You then fractionate the isolation product by size using
In order to visualize the expression of your candidate gene you transfer or blot this product onto a nitrocellulose membrane followed by incubation with a