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Cellular and Molecular Biology

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Primer Design for PCR: Melting Temperature

CELLS-1HPPFN

You are a biologist who needs to design primers to amplify a particular gene of interest using the Polymerase Chain Reaction (PCR). You must design a forward primer and a reverse primer with melting temperatures (or ${ T }_{ m }$) no more than $2℃$ apart from one another.

What determines the ${ T }_{ m }$ of a particular primer?

A

GC Content.

B

Primer Length.

C

Palindromic Sequences.

D

Secondary Structure.