You are part of a research team investigating Zika virus, and you are charged with identifying an antibody that can be used to develop a diagnostic test.
An initial literature search suggests that the Zika virus E protein, the major surface protein of the virus, is the best antigen to target with your assay. The E protein is a 53kDa protein that is highly glycosylated and transits to the surface of an infected cell through the secretory pathway.
You decide to screen a set of available antibodies against the E protein using immunofluorescence on cells infected with Zika virus. Your goal is to identify an antibody that specifically binds to the glycosylated residues of the Zika virus E protein.
You expect to see fluorescence (antibody-binding) at the surface of the infected cell, as this is the final destination of the mature protein.
Using your knowledge of the secretory pathway, predict which internal cell organelle is most likely to display fluorescence as well.