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Cellular and Molecular Biology

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Supershift of DNA-Protein-Antibody Complexes

CELLS-6NJJSB

In this electrophoresis mobility shift assay (EMSA), a strand of DNA was incubated with a protein in the left lane and with the same protein and an antibody to that protein in the right lane.

Which ONE of the descriptions and explanations below is correct?

Celeste R. Wirsig-Wiechmann – Created for Albert.io. Copyright 2016. All rights reserved.

A

‘B’ represents the unbound DNA while 'C' and 'A' represent DNA with bound protein and with bound protein and antibody, respectively. This experiment confirms that the protein binds to the DNA but that the antibody does not bind to DNA.

B

‘A’ represents the unbound DNA, 'B' represents DNA with bound protein and 'C' represents DNA with bound antibody. This experiment confirms that the DNA binds both the protein and the antibody.

C

‘A’ represents the unbound DNA, 'C' represents DNA with bound protein, and 'B' represents DNA with bound protein which in turn is bound by the antibody. This experiment confirms that the protein binds to the DNA since the antibody binding to the protein shifts the position of the bound DNA.

D

'C' represents unbound DNA, 'B' represents DNA bound by the protein and 'A' represents DNA with neither protein nor antibody bound to it. This experiment confirms that the protein can bind DNA but then dissociate.