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Cellular and Molecular Biology

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Transgene Expression Strategy

CELLS-WA@KJG

By use of restriction endonucleases, you cut the human insulin gene (DNA) from β cell of the Islets of Langerhans and insert it into the bacterium Escherichia coli.

The E. coli then expresses the desired protein. Unfortunately, you find out that the insulin produced is non-functional and has many more amino acids than does the corresponding and functional human insulin.

Given that there are no mutations in the human DNA used, what strategy would you use for BEST resolving this problem?

A

Isolate mRNA for insulin synthesis from the β cells of the human pancreas and use reverse transcriptase to convert it into complementary DNA (cDNA) and insert the cDNA into bacterium.

B

Isolate the gene for insulin synthesis from the β cells of the human pancreas and insert the DNA into the bacterium.

C

Isolate all the mRNA from the cells of the human pancreas and use reverse transcriptase to convert it into complementary DNA (cDNA) and insert the cDNA into the bacterium.

D

Isolate the primary mRNA transcript for insulin synthesis from the β cells of the human pancreas and use reverse transcriptase to convert it into complementary DNA (cDNA) and insert the cDNA into the bacterium.