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Cellular and Molecular Biology

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Using Radioisotopes to Track Respiration

CELLS-B2OBJ6

Mitochondria are the organelles in which energy stored in foods is released to power cellular activities. Aerobic respiration is the biochemical pathway used under normal conditions (oxygen being present). In respiration, glucose (containing high-energy carbon-hydrogen bonds) is broken down, which powers production of ATP.

Which of the following choices is the BEST laboratory approach to trace the biochemical pathway of carbon utilization in aerobic respiration?

A

Radioactively tag oxygen breathed by a rat after consuming a meal.

B

Radioactively tag nitrogen in food given to a rat.

C

Radioactively tag hydrogen in food given to a rat.

D

Radioactively tag carbon in food given to a rat.