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Cellular and Molecular Biology

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Visual Analysis of Cell Culture via Microscopy

CELLS-JOEEL6

When you observe your neural stem cell culture with the phase contrast microscope, you see the following image:

Elsbernd, Joseph. Neuronal Stem Cells. Digital image. Flckr. Flckr Creative Commons, 24 May 2011. Web. 25 Feb. 2016.

Which statement best explains the small, bright, spherical structures visible in the culture?

A

The spherical structures are smaller than the stem cells, indicating that they are microbial contamination.

B

The spherical structures are cells undergoing cell division and have detached from the surface of the plate.

C

The spherical structures represent the undifferentiated stem cells and the larger cells with distinctive shape represent cells that have differentiated in this culture.

D

The spherical structures represent components of the growth media that have precipitated out of solution.