Genetics and Genomics

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Chromatin State and Eukaryotic Expression


While working with cultured female mammalian cells, you transfect them using a tailored retrovirus to insert a gene of interest. After screening, you find four cells with your gene of interest integrated into the genome, each in a different location.

Which location would be most likely to express the integrated gene?

The chromatin state (how open or folded the chromatin is) affects both the likelihood​ of integration and how highly it is expressed, with more open chromatin having higher expression.


The gene has integrated into the X chromosome.


The gene has integrated near the centromere.


The gene has integrated into intergenic space near metabolic genes.


The gene has integrated into intergenic space near genes involved in meiosis.