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# Determining Complementation Groups in Auxotrophic Yeast

GENES-A8YFHB

You are working with a set of auxotrophic mutant yeast (mutants unable to synthesize amino acids or nucleotides from minimal media). These mutant strains are labeled 1 through 7. You cross the yeast to determine their complementation groups. Complementation groups are groups of mutants that are unable to complement each other.

Mutants were made in both mating types of yeast and crossed to form diploid yeast. These diploids were then plated on minimal media.

The results of the crosses are as follows:

1 2 3 4 5 6 7
1 - + - - + + -
2 + - + + - + +
3 - + - - + + -
4 - + + - + + -
5 + - + + - + +
6 + + + + + - +
7 - + - - + + -

$\$
Using this data, you are able to make the following conclusions (it may be helpful to work this problem on paper).

The largest complementation group consists of mutants

,

,

and

(be sure to list them from the lowest number to highest).

The next largest complementation group consists of mutants

and

(be sure to list them from the lowest number to highest).

The final complementation group is mutant

.

Genes that are in the same complementation group likely have mutations in the same

.