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Odd Results In a Mutant Complementation Test

GENES-SYIIBJ

While working with a series of yeast loss of function mutants, you perform a complementation test by mating each haploid yeast mutant with every other (mutants are labeled A-E) as well as mating them with wild-type (WT) haploid. The results of testing the resulting diploids are as follows: a plus mark indicates there was complementation, and a minus mark means there was no complementation.

WT A B C D E
WT + + - + + +
A + - - - + +
B - - - - - -
C + - - - + +
D + + - + - -
E + + - + - -

The mutant that stands out as unusual is mutant

.

The other four mutants fall into two complementation groups. Listed alphabetically, they are

and

.

The purpose of mating the mutants to the wild-type is to allow you to determine if the mutants are

.

The unusual mutant in the first dropdown would be best described as

.