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Genetics and Genomics

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The Functional Importance of Primers in PCR

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Which of the following are reasons that primers are necessary for the function of PCR?

Select ALL that apply.

A

Primers provide DNTPs (the four types of nucleotide triphosphates) for the construction of the newly replicated DNA.

B

Primers set the boundaries​ and locations of where DNA will be replicated within the template DNA strand.

C

Primers set the points where the DNA strand will be cut.

D

RNA polymerase requires a strand to elongate and cannot synthesize a strand de novo.

E

DNA polymerase requires a strand to elongate and cannot synthesize a strand de novo.

F

Primers serve as a binding site for the RNA machinery.