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Which of the following are reasons that primers are necessary for the function of PCR?
Select ALL that apply.
Primers provide DNTPs (the four types of nucleotide triphosphates) for the construction of the newly replicated DNA.
Primers set the boundaries and locations of where DNA will be replicated within the template DNA strand.
Primers set the points where the DNA strand will be cut.
RNA polymerase requires a strand to elongate and cannot synthesize a strand de novo.
DNA polymerase requires a strand to elongate and cannot synthesize a strand de novo.
Primers serve as a binding site for the RNA machinery.