Signaling centers known as secondary organizers promote specification of the three domains in the anterior neural tube: prosencephalon or forebrain, mesencephalon or midbrain and rhombencephalon or hindbrain.
Anterior neural ridge (ANR) is one such center. It is located between the most rostral part of the neural plate and non-neural ectoderm. Molecules produced and released by ANR, primarily fibroblast growth factor 8 (Fgf8), promote specification and proliferation of anterior prosencephalic structures.
Which of the following is the CORRECT experimental manipulation and expected outcome pair?