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Organic Chemistry 2

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Moderate

Polymerase Chain Reaction: Annealing Step

ORGO2-NXIM$7

Which of the following statements is (are) correct about the annealing step of PCR?​

Select ALL that apply.

A

Annealing is the first step in PCR.

B

Annealing takes place at a higher temperature than denaturation.

C

Annealing requires not very high and not very low temperatures.

D

If the temperature is too low, the primer binds non-specifically.​

E

If the temperature is too high, the primer does not bind. ​

F

The annealing temperature should be below the $ { T }_{ m }$ of the primer used.