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2,4-dinitrophenol, or DNP, is a chemical that was used as a weight loss aid until it was banned in the 1930s. When it reacts with hydrogen ions and become pronated, DNP becomes hydrophobic and can easily diffuse directly through lipid bilayer membranes.

Inside of a mitochondrion, which of the following is the direct consequence of exposure to the chemical DNP?


ATP synthase's affinity for hydrogen ions is decreased.


The ability of Protein Complex I to oxidize NADH molecules is enhanced.


The organelle's ability to establish and maintain a proton gradient is decreased.


The production of carbonic acid in the mitochondrial matrix is increased.

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