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PCR is a technique used to amplify a certain segment of DNA, potentially creating millions of copies.

Which of the following describes a key step in performing PCR?


Heat shock at 42 Celsius for 90 seconds.


Addition of $CaCl_2$ to make the cell’s DNA.


Addition of Taq polymerase to replicate the DNA.


Adding RNA primers to flank the ends of the DNA that are targeted for copying.

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