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Ribulose-1,5-bisphosphate carboxylase/oxygenase is an enzyme that catalyzes the first committed step in carbon fixation and is commonly known by the abbreviation rubisco, or more properly, RuBisCO. Prior to catalysis, rubisco must first be activated.

Which of the following is NOT required for activation of rubisco?

A

ATP hydrolysis.

B

Binding of $Mg^{2+}$.

C

Carbamoylation of the active site lysine.

D

Ribulose 1,5-bisphosphate in the active site.

E

Release of 2-carboxyarabinitol from the active site.

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