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Arginase is an enzyme that catalyzes the formation of ornithine from arginine. When the enzyme assay is done for arginase, the instructions call for mixing the enzyme with manganese chloride for 10 minutes and then portions of an arginine solution and a glycine solution adjusted to pH 9.0 are added for 30 minutes (at which time perchloric acid is added).

Each of these components plays a different role in the enzyme assay.

Manganese chloride
Select Option is a substratemaintains a constant pH for the enzymeis a required co-factorstops the reaction by denaturing the enzyme
, while arginine
Select Option is a substratestops the reaction by denaturing the enzymeis a required co-factormaintains a constant pH for the enzyme
. The glycine pH 9.0 solution serves
Select Option as a required co-factorto maintain a constant pH for the enzymeto stop the reaction by denaturing the enzymeas a substrate
, and the perchloric acid
Select Option maintains a constant pH for the enzymeis a substrateis a required co-factorstops the reaction by denaturing the enzyme
.
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