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What is true about the process used in the chemical synthesis of oligonucleotides?

Select ALL that apply.

A

The reaction of each nucleotide is done manually.

B

The finished oligonucleotide is generally less than 100 bases in length.

C

The growing oligonucleotide is assembled by using the 3' end of the terminal oligonucleotide to bind to the 5' end of the next, incoming nucleotide.

D

Only the four bases nucleotide bases present in DNA may be a part of the synthesized sequence.

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