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Substrate-level phosphorylation of ADP to ATP during glycolysis generates a total of four ATP. In the enzyme reactions that generate ATP during glycolysis, substrates bind to their respective enzymes causing conformational changes between the enzyme and substrates. These conformational changes lower the activation energy of the reactions leading to the transfer of a phosphoryl group to ADP, resulting in the production of ATP.

Which of the following enzymes would most likely play a role in this reaction?

A

Kinase.

B

Phosphatase.

C

Hydrolase.

D

Endonuclease.

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