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During a 12-week experiment, male rats were treated with several different hormones. Necropsies were then performed to determine the effects of the hormones on their glands and body mass. Some of the rats were castrated to eliminate the androgen production for comparison. The data below represents the experimental findings.

Non-Castrated Rats

Variable Control Rat Pair Experimental Rat Pair 1
Pituitary 11.8 mg 9.2 mg
Thyroid 245 mg 248 mg
Thymus 475 mg 470 mg
Adrenals 42 mg 40 mg
Seminal Vesicle 500 mg 1300 mg
Prostate 425 mg 900 mg
Testes 3000 mg 2200 mg
Body Mass 305 g 200 g



Castrated Rats

Variable Control Rat Pair Experimental Rat Pair 1
Pituitary 11.8 mg 9.1 mg
Thyroid 245 mg 245 mg
Thymus 480 mg 470 mg
Adrenals 42 mg 43 mg
Seminal Vesicle 475 mg 1250 mg
Prostate 387 mg 800 mg
Testes removed removed
Body Mass 275 g 490 g



Calculate the percentage of difference between the control rats and the experimental rats. Note: Only differences greater than 20% should be considered significant.

Based on the gland and body mass changes, which hormone was likely given to Rat Pair 1?

A

Adrenocorticotropic hormone.

B

Luteinizing hormone.

C

Cortisol.

D

Testosterone.

E

Estrogen.

F

Thyroid stimulating hormone.

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