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Which of the following statements regarding the separation of DNA molecules by agarose gel electrophoresis is TRUE?

Select ALL that apply.

A

DNA is negatively charged and moves towards a positive electrode.

B

DNA is positively charged and moves towards a negative electrode.

C

Larger DNA molecules move farther through an agarose gel than smaller molecules.

D

DNA molecules with higher GC content move farther through an agarose gel than those with lower GC content.

E

Smaller DNA molecules move farther through an agarose gel than larger molecules.

F

DNA molecules with lower GC content move farther through an agarose gel than those with higher GC content.

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