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An enzyme assay was done with substrate alone (black line), a 50 nM concentration of competitive inhibitor X (red line), and competitive inhibitor Y (blue line). The results were used to construct a Lineweaver-Burk plot, which is shown. ${ K }_{ I }$ is a term that describes the strength of inhibitor binding to the enzyme; it is the equilibrium constant for this reaction:

$$EI \xrightarrow [ ]{ } E+I$$

Which of the following contains a TRUE statement about the ${ K }_{ I }$ for inhibitors X and Y?

The y-axis is 1/Velocity and the x-axis is 1/[substrate]. Kelly Keenan. Created for Copyright 2016. All rights reserved


Inhibitor X has a larger value for ${ K }_{ I }$ than Y.


Inhibitor X has the same value for ${ K }_{ I }$ as Y.


Inhibitor X has a smaller value for ${ K }_{ I }$ than Y.


It is not possible to determine the relative values of ${ K }_{ I }$.

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