Limited access

An enzyme assay was done with substrate alone (black line), a 50 nM concentration of competitive inhibitor X (red line), and competitive inhibitor Y (blue line). The results were used to construct a Lineweaver-Burk plot, which is shown. ${ K }_{ I }$ is a term that describes the strength of inhibitor binding to the enzyme; it is the equilibrium constant for this reaction:

$$EI \xrightarrow [ ]{ } E+I$$

Which of the following contains a TRUE statement about the ${ K }_{ I }$ for inhibitors X and Y?

A

Inhibitor X has a larger value for ${ K }_{ I }$ than Y.

B

Inhibitor X has the same value for ${ K }_{ I }$ as Y.

C

Inhibitor X has a smaller value for ${ K }_{ I }$ than Y.

D

It is not possible to determine the relative values of ${ K }_{ I }$.

Select an assignment template