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Myoglobinuria is a rare, genetically transmitted disease caused by mutations in the LPIN1 gene, which encodes the phosphatidate phosphatase enzyme, and the affected protein exists as a single subunit. This gene does show evidence of being transmitted in an autosomal recessive pattern.

Which of the following describes the level(s) of protein structure disrupted by this mutation that produces myoglobinuria?

I. Primary (${ 1 }^{ ° }$) structure.
II. Tertiary (${ 3 }^{ ° }$) structure.
III. Quaternary (${ 4 }^{ ° }$) structure.


I and II.


I and III.


II and III.


I, II, and III.

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